doi: 10.1093/jexbot/52.356.641.
Nuclear magnetic resonance micro-imaging in the investigation of plant cell metabolism
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- PMID: 11373312
- DOI: 10.1093/jexbot/52.356.641
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Nuclear magnetic resonance micro-imaging in the investigation of plant cell metabolism
J Exp Bot.
2001 Apr.
Abstract
Micro-imaging based on nuclear magnetic resonance offers the possibility to map metabolites in plant tissues non-invasively. Major metabolites such as sucrose and amino acids can be observed with high spatial resolution. Stable isotope tracers, such as (13)C-labelled metabolites can be used to measure the in vivo conversion rates in a metabolic network. This review summarizes the different nuclear magnetic resonance micro-imaging techniques that are available to obtain spatially resolved information on metabolites in plants. A short general introduction into NMR imaging techniques is provided. Particular emphasis is given to the difficulties encountered when NMR micro-imaging is applied to plant systems.
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