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. 2001 Jun;67(6):2840-3.
doi: 10.1128/AEM.67.6.2840-2843.2001.

Quantitative shedding of two genotypes of Cryptosporidium parvum in California ground squirrels (Spermophilus beecheyi)

E R Atwill  1 , S M Camargo, R Phillips, L H Alonso, K W Tate, W A Jensen, J Bennet, S Little, T P Salmon
Affiliations

Quantitative shedding of two genotypes of Cryptosporidium parvum in California ground squirrels (Spermophilus beecheyi)

E R Atwill et al. Appl Environ Microbiol. 2001 Jun.

Abstract

Sixteen percent of California ground squirrels (Spermophilus beecheyi) were found to be shedding an average of 53,875 Cryptosporidium parvum oocysts/g of feces. Male squirrels had a higher prevalence and higher intensity of shedding than did female squirrels. The majority of C. parvum isolates matched a bovine-murine genotype, with a few isolates resembling a porcine genotype. Higher intensities of shedding by males may enhance dissemination and genotypic mixing of this protozoa given males' proclivity to disperse to nonnatal colonies.

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Figures

FIG. 1
FIG. 1
Confirmation of species and genotyping of C. parvum oocysts from California ground squirrels through one-step PCR-RFLP and nested PCR-RFLP analysis. Lane 1, 100- to 1,500-bp markers; lane 2, 550-bp amplicon from COWP gene primers; lane 3, 410 and 106-bp fragments from COWP RFLP analysis with RsaI; lane 4, 1,325-bp amplicon from SSU rRNA primary product; lane 5, 831-bp amplicon from SSU rRNA secondary product, genotype bovine B; lane 6, 838-bp amplicon from SSU rRNA secondary product, genotype porcine; lane 7, 628-bp and 102- to 104-bp fragments from porcine genotype with VspI; lane 8, 453- and 365-bp fragments from porcine genotype with SspI; lane 9, 449-, 254-, and 119-bp fragments from genotype bovine B with SspI; lane 10, 628-bp and 102- to 104-bp fragments from genotype bovine B with VspI; lane 11, 100- to 1,500-bp markers.
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