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. 2001 Jun;67(6):2859-62.
doi: 10.1128/AEM.67.6.2859-2862.2001.

Persistence of a Salmonella enterica serovar typhimurium DT12 clone in a piggery and in agricultural soil amended with Salmonella-contaminated slurry

S B Baloda  1 , L Christensen, S Trajcevska
Affiliations

Persistence of a Salmonella enterica serovar typhimurium DT12 clone in a piggery and in agricultural soil amended with Salmonella-contaminated slurry

S B Baloda et al. Appl Environ Microbiol. 2001 Jun.

Abstract

Prevalence of Salmonella enterica on a Danish pig farm presenting recurrent infections was investigated. A comparison of the pulsed-field gel electrophoresis patterns of fecal isolates from piggeries, waste slurry, and agricultural soil amended with Salmonella-contaminated animal waste (slurry) and subclinical isolates from the same farm (collected in 1996 and later) showed identical patterns, indicating long-term persistence of the Salmonella enterica serovar Typhimurium DT12 clone in the herd environment. Furthermore, when Salmonella-contaminated slurry was disposed of on the agricultural soil (a common waste disposal practice), the pathogen was isolated up to 14 days after the spread, indicating potentially high risks of transmission of the pathogen in the environment, animals, and humans.

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Figures

FIG. 1
FIG. 1
PFGE typing of selected Salmonella serovar Typhimurium strains isolated from piggeries, slurry, and soil amended with Salmonella-contaminated slurry (treated soil). Lane 1 contains molecular size markers (kilobases) (Lambda Ladder PFG marker; New England Biolabs). Lanes 2 to 5 contain DNA from selected Salmonella serovar Typhimurium isolates from piggeries, slurry, and agricultural soil amended with Salmonella-contaminated slurry (i.e., treated soil), as follows: lane 2, isolate S 83—piggery sample (tested on 10 March 2000); lane 3, isolate S 86—slurry sample (tested before being spread on agriculture soil on 11 March 2000); lane 4, isolate S 88—treated soil (sampled on 11 March 2000); lane 5, isolate S 114—treated soil sampled 14 days after spreading of slurry. All serovar Typhimurium DNA samples shown were digested with the restriction enzyme BlnI.
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