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Comparative Study
. 2001 Jun;39(6):2283-6.
doi: 10.1128/JCM.39.6.2283-2286.2001.

Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals

J A Wagenaar  1 , M A van Bergen, D G Newell, R Grogono-Thomas, B Duim
Affiliations
Comparative Study

Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals

J A Wagenaar et al. J Clin Microbiol. 2001 Jun.

Abstract

A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination methods were unreliable. Genotyping of the strains by PCR and AFLP showed a clear discrimination between the two subspecies.

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Figures

FIG. 1
FIG. 1
Dendrogram showing the AFLP banding patterns of 69 C. fetus strains. Cluster analysis was based on the similarity levels among bands in region 841 to 879 of the banding patterns (arrow). The different clusters of C. fetus subsp. fetus and C. fetus subsp. venerealis are indicated. The percentages of genetic similarity among banding patterns are shown.
See this image and copyright information in PMC

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