Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2001 Jun;39(6):2306-7.
doi: 10.1128/JCM.39.6.2306-2307.2001.

Comparison of a polymer conjugate-enhanced enzyme immunoassay to ligase chain reaction for diagnosis of Chlamydia trachomatis in endocervical swabs

M Chernesky  1 , D Jang, D Copes, J Patel, A Petrich, K Biers, A Sproston, J Kapala
Affiliations
Comparative Study

Comparison of a polymer conjugate-enhanced enzyme immunoassay to ligase chain reaction for diagnosis of Chlamydia trachomatis in endocervical swabs

M Chernesky et al. J Clin Microbiol. 2001 Jun.

Abstract

Two endocervical swabs from each of 1,123 women were collected into manufacturer-supplied transport tubes and tested for Chlamydia trachomatis by a polymer conjugate-enhanced (PCE) enzyme immunoassay (EIA) (IDEIA PCE Chlamydia; DAKO) and a ligase chain reaction assay (LCx Chlamydia; Abbott). After confirmation by the EIA blocking test, the sensitivity of the IDEIA PCE remained at 91.8% and the specificity increased from 98.2 to 99.8% compared to LCx.

PubMed Disclaimer

References

    1. Chernesky M A, Jang D, Lee H, Burczak J D, Hu H, Sellors J, Tomazic-Allen S J, Mahony J B. Diagnosis of Chlamydia trachomatis infections in men and women by testing first-void urine by ligase chain reaction. J Clin Microbiol. 1994;32:2682–2685. - PMC - PubMed
    1. Chernesky M A, Lee H, Schachter J, Burczak J D, Stamm W E, McCormack W M, Quinn T C. Diagnosis of Chlamydia trachomatis urethral infection in symptomatic and asymptomatic men by testing first-void urine in a ligase chain reaction assay. J Infect Dis. 1994;170:1308–1311. - PubMed
    1. Clarke L M, Sierra M F, Daidone B J, Lopez N, Covino J M, McCormack W M. Comparison of the Syva MicroTrak enzyme immunoassay and Gen-Probe PACE 2 with cell culture for diagnosis of cervical Chlamydia trachomatis infection in a high-prevalence female population. J Clin Microbiol. 1993;31:968–971. - PMC - PubMed
    1. Hook E W, III, Smith K, Mullen C, Stephens J, Rinehardt L, Pate M S, Lee H H. Diagnosis of genitourinary Chlamydia trachomatis infections by using the ligase chain reaction on patient-obtained vaginal swabs. J Clin Microbiol. 1997;35:2133–2135. - PMC - PubMed
    1. Launderdale T-L, Landers L, Thorneycroft I, Chapin K. Comparison of the PACE 2 assay, two amplification assays, and Clearview enzyme immunoassay for detection of Chlamydia trachomatis in female endocervical and urine specimens. J Clin Microbiol. 1999;37:2223–2229. - PMC - PubMed

MeSH terms

Substances