Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Jun;39(6):2335-7.
doi: 10.1128/JCM.39.6.2335-2337.2001.

Amplified fragment length polymorphism fingerprinting for identification of a core group of Neisseria gonorrhoeae transmitters in the population attending a clinic for treatment of sexually transmitted diseases in Amsterdam, The Netherlands

J Spaargaren  1 , J Stoof, H Fennema, R Coutinho, P Savelkoul
Affiliations

Amplified fragment length polymorphism fingerprinting for identification of a core group of Neisseria gonorrhoeae transmitters in the population attending a clinic for treatment of sexually transmitted diseases in Amsterdam, The Netherlands

J Spaargaren et al. J Clin Microbiol. 2001 Jun.

Abstract

Amplified fragment length polymorphism analysis seems well suited for studying the epidemiology of isolates of Neisseria gonorrhoeae obtained from patients attending the Sexually Transmitted Disease Outpatient Clinic in Amsterdam, The Netherlands. It shows potential to identify the core group of transmitters.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Fluorescently labeled AFLP patterns and dendrogram of 29 strains, including 22 clinical N. gonorrhoeae isolates, obtained after amplification of EcoA and MseO templates. The patterns were analyzed by Pearson correlation, and a dendrogram was constructed with the UPGMA (unweighted pair group method with averages) BioNumerics software package (Applied Maths, Kortrijk, Belgium). Percentages of similarity are shown above the dendrogram. The strain numbers and patient codes are shown on the right. Known sexual contacts are indicated by asterisks, plus signs, and quotation marks. ATCC 23970, N. lactamica; ATCC 13090, N. meningitidis; ATCC 25238, Moraxella catarrhalis. 3 Antw., 1979-Thailand; 8 Antw., 1989-United Kingdom; 11 Antw., 1993-United Kingdom; 18 Antw., 1989-Gambia.
See this image and copyright information in PMC

References

    1. Brunham R C. The concept of core and its relevance to the epidemiology and control of sexually transmitted diseases. Sex Transm Dis. 2000;18:67–68. - PubMed
    1. Camarena J J, Nogueira J M, Dasi M A, Moreno F, Garcia R, Ledesma E, Llorca J, Hernandez J. DNA amplification fingerprinting for subtyping Neisseria gonorrhoeae strains. Sex Transm Dis. 1995;22:128–136. - PubMed
    1. Fennema J S, Cairo I, Coutinho R A. Substantial increase in gonorrhea and syphilis among clients of Amsterdam Sexually Transmitted Diseases Clinic. Ned Tijdschr Geneeskd. 2000;144:602–603. - PubMed
    1. Isenberg H D, editor. Clinical microbiology procedures handbook. Washington, D.C.: American Society for Microbiology; 1992.
    1. Koeleman J G, Stoof J, Biesmans D J, Savelkoul P H, Vandenbroucke-Grauls C M. Comparison of amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism fingerprinting for identification of Acinetobacter genomic species and typing of Acinetobacter baumannii. J Clin Microbiol. 1998;36:2522–2529. - PMC - PubMed

LinkOut - more resources