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. 2001 Jun;39(6):2344-7.
doi: 10.1128/JCM.39.6.2344-2347.2001.

Sampling variability in the microbiological evaluation of expectorated sputa and endotracheal aspirates

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Sampling variability in the microbiological evaluation of expectorated sputa and endotracheal aspirates

S Nagendra et al. J Clin Microbiol. 2001 Jun.

Abstract

A five-center study was conducted with the aim of determining how reproducibly expectorated sputa and tracheal aspirates could be sampled when preparing Gram-stained smears and inoculating cultures. With both specimen types, excessive variation was noted among Gram stain results obtained from replicate smears. Less variation was noted among culture results, especially with tracheal aspirates.

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