Comparative Study
doi: 10.1089/10665270050514963.
Fidelity of enzymatic ligation for DNA computing
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- PMID: 11382365
- DOI: 10.1089/10665270050514963
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Comparative Study
Fidelity of enzymatic ligation for DNA computing
J Comput Biol.
2000.
Abstract
We describe a convenient assay for rapid qualitative evaluation of hybridization/ligation fidelity. The approach uses randomized probe strands of DNA and restriction enzyme digestion after amplification of reaction products by the polymerase chain reaction (PCR). We report ligation efficiencies and fidelities of two DNA ligases, T4 DNA ligase and Thermus aquaticus (Taq) DNA ligase, over a range of temperatures.
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