Effects of thyroid hormones on bovine granulosa and thecal cell function in vitro: dependence on insulin and gonadotropins

Abstract

The objective of this study was to evaluate the influence of thyroxine (T4) and triiodothryonine (T3) on steroid production by bovine granulosa and thecal cells. Granulosa and thecal cells were obtained from small (1 to 5 mm) and large (> or = 8 mm) follicles of cattle, respectively, and cultured for 4 d. We conducted six experiments to evaluate the effect of 2 d of exposure to various doses of T3 or T4. In insulin- or insulin plus FSH-treated granulosa cells of experiment 1, 30 and 100 ng/ml of T4 had no effect on aromatase activity or progesterone production. In experiment 2, in the presence of insulin and FSH, 1 and 3 ng/ml of T3 weakly (<1.4-fold) increased aromatase activity of granulosa cells but had no effect on progesterone production. Low doses of T4 (3 to 30 ng/ml) tested in experiment 3 had no effect on aromatase activity but increased (to as much as 1.4-fold) progesterone production by granulosa cells. In experiment 4, T4 (30 ng/ml) increased (to 1.2-fold) progesterone production by granulosa cells only in the presence of FSH and had no effect on aromatase activity. In thecal cells of experiment 5, in the presence of insulin and LH, 30 and 100 mg/ml of T4 increased androstenedione production to 2.3- and 2.8-fold, respectively; only 100 ng/ml of T4 was effective at stimulating progesterone production by thecal cells. In experiment 6, 1 ng/ml of T3 increased thecal cell androstenedione production to 3.9-fold, whereas 3 ng/ml of T3 was without effect; progesterone production was not affected by T3. These results support the hypothesis that thyroid hormones may have direct stimulatory effects on ovarian function in cattle, acting at the level of granulosa and thecal cells.