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. 2001 May 3;119(3):114-8.
doi: 10.1590/s1516-31802001000300006.

Paragangliomas of the head and neck: clinical, morphological and immunohistochemical aspects

P A Filho  1 , A Rapoport, V A Alves, O V Denardin, J Sobrinho, M B Carvalho
Affiliations

Paragangliomas of the head and neck: clinical, morphological and immunohistochemical aspects

P A Filho et al. Sao Paulo Med J. .

Abstract
in English, Portuguese

Context: Protein marker positivity can assist in the definition of the therapeutic approach towards head and neck paragangliomas. The establishment of the therapeutic approach should incorporate the results of such an investigation.

Objective: To establish criteria for benignity and malignancy of vagal and jugular-tympanic paragangliomas, via the study of the relationships of sex, age, tumor size, duration of complaints, site, family history, presence of metastases, treatment, histological architecture and cell type with the immunohistochemical reactions to S100 protein, chromogranin and AgKi67.

Design: A retrospective study of histological and clinical records.

Setting: The Heliópolis and Oswaldo Cruz tertiary general hospitals, São Paulo.

Sample: 8 cases of head and neck paragangliomas.

Main measurements: Determination of degree of positivity to paragangliomas via immunohistochemical reactions.

Results: 1). The protein markers for the principal cells (AgKi67 and chromogranin) were sensitive in 100% of the tumors when used together. 2). S100 protein was well identified in the cytoplasm and nucleus of sustentacular cells and underwent reduction in the neoplasias.

Conclusions: Chromogranin was proven to be a generic marker for neuroendocrine tumors; S100 protein was positive in all 8 cases and the AgKi67 had low positivity in all cases.

CONTEXTO:: . A marcação positiva pode auxiliar na escolha da terapêutica dos paragangliomas de cabeça e pescoço. A definição do tratamento deve ser realizada incorporando o resultado desses testes.

OBJETIVO:: Por meio do estudo da relação do sexo, idade, tamanho de lesão, tempo de queixa, localização, história familiar, metástase, tratamento, arquitetura, tipo celular com as reações imunohistoquímicas para proteína S100, cromogranina e AgKi67, estabelecimento de critérios de benignidade e malignidade dos paragangliomas vagais e júgulo-timpânicos.

TIPO DE ESTUDO:: Estudo retrospectivo de arquivos histológicos e clínicos.

LOCAL:: Hospital Heliópolis e Oswaldo Cruz, São Paulo.

PARTICIPANTES:: Oito casos de paragangliomas de cabeça e pescoço.

VARIÁVEIS ESTUDADAS:: Grau de positividade para paragangliomas com reação imunohistoquímica.

RESULTADOS:: Os marcadores protéicos para as células principais (AgKi67 e cromogranina) foram sensíveis em 100% das neoplasias quando usados conjuntamente; A proteína S100 foi bem identificada no citoplasma e núcleo das células sustentaculares e sofre diminuição nas neoplasias.

CONCLUSÕES:: a cromogranina comprovou ser um marcador genérico para os tumores neuroendócrinas, a proteína S100 foi positiva nos oito casos e o Ki67 foi baixo em todos os casos.

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Conflict of interest statement

Conflict of interest: Not declared

Figures

Figure 1
Figure 1. Cells stained by Cromogranin.
Figure 2
Figure 2. Sustentocullar cells stained by S100 protein.
Figure 3
Figure 3. AgKi67 staining two nucleous.
See this image and copyright information in PMC

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