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. 2001 Jul;69(7):4695-7.
doi: 10.1128/IAI.69.7.4695-4697.2001.

Hyphae and yeasts of Candida albicans differentially regulate interleukin-12 production by human blood monocytes: inhibitory role of C. albicans germination

L Liu  1 , K Kang, M Takahara, K D Cooper, M A Ghannoum
Affiliations

Hyphae and yeasts of Candida albicans differentially regulate interleukin-12 production by human blood monocytes: inhibitory role of C. albicans germination

L Liu et al. Infect Immun. 2001 Jul.

Abstract

The role of Candida albicans yeast-to-hyphae transition in interleukin-12 (IL-12) production by monocytes was investigated. Germinating C. albicans not only failed to induce IL-12 p70 but also suppressed IL-12 production induced by heat-killed C. albicans. Comparison of the abilities of germinating C. albicans and agerminating mutants to inhibit IL-12 production showed that germination of C. albicans plays a critical role in the inhibition of IL-12 production.

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Figures

FIG. 1
FIG. 1
Live C. albicans (L-CA) inhibits heat-killed (HK) C. albicans-induced IL-12 production by MN. IL-12 p70 levels are expressed as means ± the standard errors of the means. The ratios 1:10 and 3:10 represent the C. albicans-to-MN ratios used. n = 4 to 10.
FIG. 2
FIG. 2
Viability of monocytes cocultured with different ratios of C. albicans (CA). Fluorescence intensity represents MN viability as determined by cytofluorometer. The ratios 1:10, 3:10, and 1:2 represent the C. albicans-to-MN ratios used. n = 2.
FIG. 3
FIG. 3
C. albicans (CA) hyphae and blastospores differentially induce IL-12 production by MN. (A) IL-12 p70 levels in supernatants were measured by enzyme-linked immunosorbent assay. n = 2 to 6. (B) RT-PCR for IL-12 p40 mRNA expression by MN. The data shown represent three experiments with similar results. Refer to the text for a description of the Candida strains used. HK-CA, heat-killed C. albicans.
FIG. 4
FIG. 4
Inhibition of induced IL-12 production by C. albicans. (A) IL-12 p70 levels are expressed as means ± the standard errors of the means. n = 3 to 9. (B) RT-PCR for IL-12 p40 mRNA expression by MN. HK-CA, heat-killed C. albicans.
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