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. 1979 May;37(5):862-6.
doi: 10.1128/aem.37.5.862-866.1979.

Transfer of R factors to and between genetically marked sublines of Rhizobium japonicum

Transfer of R factors to and between genetically marked sublines of Rhizobium japonicum

L D Kuykendall. Appl Environ Microbiol. 1979 May.

Abstract

Plasmids R1822 and pRD1 of the P-1 incompatibility group, for which Rhizobium japonicum had not previously been shown to serve as host, were introduced into a strain of R. japonicum. Acquisition of R68 and R68.45 plasmids by this Rhizobium was equivocal. Transfer of R1822 from Pseudomonas aeruginosa and of pRD1 from Escherichia coli to R. japonicum was unambiguous, because the exconjugants subsequently cotransferred the three R-factor resistance determinants (kanamycin, tetracycline, and penicillin) between genetically marked sublines of strain I-110. Under optimal conditions the transfer of R1822 and pRD1 occurred at frequencies of approximately 10(-3) in plate matings of strains bearing as many as five dissimilar genetic markers. In matings with R1822 on membrane filters, recombinants were formed at incidences as high as 4%.

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References

    1. Proc Natl Acad Sci U S A. 1977 May;74(5):2076-8 - PubMed
    1. Basic Life Sci. 1977;9:421 - PubMed
    1. J Bacteriol. 1972 May;110(2):529-37 - PubMed
    1. Antimicrob Agents Chemother. 1973 Sep;4(3):248-53 - PubMed
    1. J Gen Microbiol. 1974 Sep;84(1):188-98 - PubMed

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