Procollagen assembly and secretion in embryonic chick bone
- PMID: 1141246
Item in Clipboard
Procollagen assembly and secretion in embryonic chick bone
J Biol Chem.
.
Free article
Abstract
Chick embryo skull bones incorporated radioactive proline and cystein into procollagen in short term organ culture. Pulse-chase experiments showed that individual precursor chains (pro-alpha1 and pro-alpha2) were formed first and that these were subsequently linked by disulfide bounds into trimers. Radioautography showed that labeled material was secreted 30 min after adding label to the cells, and electrophoretic analyses showed that after this time completed labeled collagen molecules appeared. Conversion from disulfide-linked procollagen to collagen proceeded in more than one step. An intermediate form consisting of shorter chains, which were still trimerically linked, was found.
Similar articles
-
Procollagen: biological scission of amino and carboxyl extension peptides.Proc Natl Acad Sci U S A. 1975 Dec;72(12):4905-9. doi: 10.1073/pnas.72.12.4905. Proc Natl Acad Sci U S A. 1975. PMID: 1061079 Free PMC article.
-
Collagen synthesis: a disulfide-linked collagen precursor in chick bone.Proc Natl Acad Sci U S A. 1973 Oct;70(10):2993-6. doi: 10.1073/pnas.70.10.2993. Proc Natl Acad Sci U S A. 1973. PMID: 4517950 Free PMC article.
-
Precursors of collagen secreted by cultured human fibroblasts.Proc Natl Acad Sci U S A. 1972 Dec;69(12):3655-9. doi: 10.1073/pnas.69.12.3655. Proc Natl Acad Sci U S A. 1972. PMID: 4509328 Free PMC article.
-
Secretion and extracellular processing of procollagen by cultured human fibroblasts.Proc Natl Acad Sci U S A. 1973 Feb;70(2):361-5. doi: 10.1073/pnas.70.2.361. Proc Natl Acad Sci U S A. 1973. PMID: 4346885 Free PMC article.
-
The structure and assembly of procollagen - a review.J Supramol Struct. 1974;2(2-4):108-20. doi: 10.1002/jss.400020206. J Supramol Struct. 1974. PMID: 4612241 Review. No abstract available.
Cited by
-
Procollagen: biological scission of amino and carboxyl extension peptides.Proc Natl Acad Sci U S A. 1975 Dec;72(12):4905-9. doi: 10.1073/pnas.72.12.4905. Proc Natl Acad Sci U S A. 1975. PMID: 1061079 Free PMC article.
-
The disulphide-bonded nature of procollagen and the role of the extension peptides in the assembly of the molecule.Biochem J. 1977 Feb 1;161(2):405-18. doi: 10.1042/bj1610405. Biochem J. 1977. PMID: 192195 Free PMC article.
-
Metabolism of rabbit skin collagen. Differences in the apparent turnover rates of type-I- and type-III-collagen precursors determined by constant intravenous infusion of labelled amino acids.Biochem J. 1979 Jul 1;181(1):75-82. doi: 10.1042/bj1810075. Biochem J. 1979. PMID: 486162 Free PMC article.
-
Designing collagens to shed light on the multi-scale structure-function mapping of matrix disorders.Matrix Biol Plus. 2023 Dec 14;21:100139. doi: 10.1016/j.mbplus.2023.100139. eCollection 2024 Feb. Matrix Biol Plus. 2023. PMID: 38186852 Free PMC article. Review.
-
Amino and carboxyl propeptides in bone collagen fibrils during embryogenesis.Cell Tissue Res. 1987 Jan;247(1):105-9. doi: 10.1007/BF00216552. Cell Tissue Res. 1987. PMID: 3829111
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
