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. 1979 Jul 1;181(1):107-10.
doi: 10.1042/bj1810107.

Assay of adenosine 3'-phosphate 5'-sulphatophosphate in hepatic tissues

K P Wong, T Yeo

Assay of adenosine 3'-phosphate 5'-sulphatophosphate in hepatic tissues

K P Wong et al. Biochem J. .

Abstract

A fluorimetric assay, based on the ability of boiled hepatic extracts to support the sulphO-conjugation of harmol, was used to demonstrate and quantify PAdoPS (adenosine 3'-phosphate 5'-sulphatophosphate) present in liver. A stoicheiometric relationship was established between the sulphate conjugate formed and the 'active sulphate' utilized. Guinea-pig, rat, mouse and rabbit livers contain 3.3, 2.9, 0.8 and 0.5 mumol of PAdoPS/100 G wet wt. respectively.

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