doi: 10.1016/s0014-5793(01)02516-9.
LytB, a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli
Affiliations
- PMID: 11418107
- DOI: 10.1016/s0014-5793(01)02516-9
Item in Clipboard
LytB, a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli
FEBS Lett.
.
Free article
Abstract
The mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis is essential in many eubacteria, plants, and the malaria parasite. Using genetically engineered Escherichia coli cells able to utilize exogenously provided mevalonate for isoprenoid biosynthesis by the mevalonate pathway we demonstrate that the lytB gene is involved in the trunk line of the MEP pathway. Cells deleted for the essential lytB gene were viable only if the medium was supplemented with mevalonate or the cells were complemented with an episomal copy of lytB.
Similar articles
-
Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate: a novel system for the genetic analysis of the 2-C-methyl-d-erythritol 4-phosphate pathway for isoprenoid biosynthesis.Biochem J. 2001 Jan 1;353(Pt 1):59-67. Biochem J. 2001. PMID: 11115399 Free PMC article.
-
GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli.J Bacteriol. 2001 Apr;183(8):2411-6. doi: 10.1128/JB.183.8.2411-2416.2001. J Bacteriol. 2001. PMID: 11274098 Free PMC article.
-
Identification of gcpE as a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis in Escherichia coli.FEBS Lett. 2001 Jan 19;488(3):170-3. doi: 10.1016/s0014-5793(00)02420-0. FEBS Lett. 2001. PMID: 11163766
-
Elucidation of the methylerythritol phosphate pathway for isoprenoid biosynthesis in bacteria and plastids. A metabolic milestone achieved through genomics.Plant Physiol. 2002 Nov;130(3):1079-89. doi: 10.1104/pp.007138. Plant Physiol. 2002. PMID: 12427975 Free PMC article. Review. No abstract available.
-
Network analysis of the MVA and MEP pathways for isoprenoid synthesis.Annu Rev Plant Biol. 2013;64:665-700. doi: 10.1146/annurev-arplant-050312-120116. Epub 2013 Mar 1. Annu Rev Plant Biol. 2013. PMID: 23451776 Review.
Cited by
-
Kinetic characterization and phosphoregulation of the Francisella tularensis 1-deoxy-D-xylulose 5-phosphate reductoisomerase (MEP synthase).PLoS One. 2009 Dec 14;4(12):e8288. doi: 10.1371/journal.pone.0008288. PLoS One. 2009. PMID: 20011597 Free PMC article.
-
Medically Useful Plant Terpenoids: Biosynthesis, Occurrence, and Mechanism of Action.Molecules. 2019 Nov 1;24(21):3961. doi: 10.3390/molecules24213961. Molecules. 2019. PMID: 31683764 Free PMC article. Review.
-
Biosynthesis pathways of ginkgolides.Pharmacogn Rev. 2013 Jan;7(13):47-52. doi: 10.4103/0973-7847.112848. Pharmacogn Rev. 2013. PMID: 23922456 Free PMC article.
-
Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein.Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1158-63. doi: 10.1073/pnas.032658999. Epub 2002 Jan 29. Proc Natl Acad Sci U S A. 2002. PMID: 11818558 Free PMC article.
-
Biosynthesis of isoprenoids: crystal structure of 4-diphosphocytidyl-2C-methyl-D-erythritol kinase.Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9173-8. doi: 10.1073/pnas.1533425100. Epub 2003 Jul 23. Proc Natl Acad Sci U S A. 2003. PMID: 12878729 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
