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. 2001 Jul;39(7):2675-6.
doi: 10.1128/JCM.39.7.2675-2676.2001.

Use of real-time quantitative PCR to detect Chlamydophila felis infection

C Helps  1 , N Reeves, S Tasker, D Harbour
Affiliations

Use of real-time quantitative PCR to detect Chlamydophila felis infection

C Helps et al. J Clin Microbiol. 2001 Jul.

Abstract

A real-time PCR assay was developed to detect and quantify Chlamydophila felis infection of cats. The assay uses a molecular beacon to specifically identify the major outer membrane protein gene, is highly reproducible, and is able to detect fewer than 10 genomic copies.

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Figures

FIG. 1
FIG. 1
Standard calibration curve for the C. felis real-time PCR assay. A C. felis PCR amplicon of known concentration was diluted from 7 × 109 molecules per 5 μl to 7 molecules per 5 μl, and 5 μl was used in the PCR assay. The threshold cycle was measured and plotted against the log10 of the starting copy number. Each point represents the average ± standard deviation for three PCRs.
FIG. 2
FIG. 2
Dilution curve of a clinical sample of C. felis DNA. Genomic DNA was isolated from a conjunctival swab taken from a cat. This was serially diluted 10-fold, and 5 μl was used in the PCR assay. The threshold cycle was measured and plotted against the log10 of the dilution. Each point represents the average ± standard deviation for three PCRs.
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