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. 2001 Apr;18(4):213-8.
doi: 10.1023/a:1009420432234.

Relationships between sperm motility characteristics assessed by the computer-aided sperm analysis (CASA) and fertilization rates in vitro

Y Hirano  1 , H Shibahara, H Obara, T Suzuki, S Takamizawa, C Yamaguchi, H Tsunoda, I Sato
Affiliations

Relationships between sperm motility characteristics assessed by the computer-aided sperm analysis (CASA) and fertilization rates in vitro

Y Hirano et al. J Assist Reprod Genet. 2001 Apr.

Abstract

Purpose: Some studies have suggested that computer-aided sperm analysis (CASA) estimates of concentration and movement characteristics of progressively motile spermatozoa are related to fertilization rates in vitro. However, it has also been suggested that the greater number of motility parameters assessed by CASA does not imply more precision in predicting fertility. This study was carried out to investigate the relationships between the CASA estimates and fertilization rates in vitro.

Methods: Semen quality analysis was performed using CASA in 136 in vitro fertilization-embryo transfer (IVF-ET) cycles with at least 3 oocytes collected. The CASA estimates before and after swim-up were compared between 108 cycles with fertilization rate > 50% ("good" group) and 28 cycles with fertilization rate < or = 50% ("poor" group).

Results: Before swim-up, there were significant correlations between fertilization rates and CASA estimates, including amplitude of lateral head displacement (ALH) (r = .269), curvilinear velocity (VCL) (r = .297), straight line velocity (VSL) (r = .266), and rapid sprm movement (Rapid) (r = .243). There was also a significant correlation between the fertilization rates and straightness (STR) after swim-up (r = -0.178). As for sperm movement characteristics, there were significant differences of ALH (p < .005), VCL (p < .001), VSL (p < .005), and Rapid (p < .01) between "good" and "poor" groups before swim-up. After swim-up, there were significant differences of VCL (p < .005), average path velocity (VAP) (p < .005), and Rapid (p < .05) between the two groups.

Conclusions: These results indicate that some of the CASA estimates provide reliable estimation of the fertilizing ability of human sperm. There were significant differences of the two sperm movement characteristics, including VCL and Rapid (before and after swim-up), indicating that the total distance traveled by rapid sperm movement might be important in human sperm fertilizing abilities.

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References

    1. Smith KD, Rodriguez-Rigau LJ, Steinberger E. Relation between indices of semen analysis and pregnancy rate in infertile couples. Fertil Steril. 1977;28:1314–1319. - PubMed
    1. Aitken RJ, Best FS, Warner P, Templeton A. A prospective study of the relationship between semen quality and fertility in cases of unexplained infertility. J Androl. 1984;5:297–303. - PubMed
    1. Irvine DS, Aitken RJ. Predictive value of in-vitro sperm function tests in the context of an AID service. Hum Reprod. 1986;8:539–545. - PubMed
    1. Ducot B, Spira A, Feneux D, Jouannet P. Male factors and the likelihood of pregnancy in infertile couples. II. Study of clinical characteristics-practical consequences. Int J Androl. 1988;11:395–404. - PubMed
    1. Bostofte E, Bagger P, Michael A, Stakemann G. Fertility prognosis for infertile men: Results of follow-up study of semen analysis in infertile men from two different populations evaluated by the Cox regression model. Fertil Steril. 1990;54:1100–1106. - PubMed