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Review
. 2001 Jul 2;20(13):3293-7.
doi: 10.1093/emboj/20.13.3293.

Control of chromosomal DNA replication in the early Xenopus embryo

J J Blow  1
Affiliations
Review

Control of chromosomal DNA replication in the early Xenopus embryo

J J Blow. EMBO J. .
No abstract available

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Figures

None
Fig. 1. Three systems required for the precise duplication of chromosomal DNA replication. Top panel, a small segment of DNA carrying three replication origins passing through the cell cycle. Red boxes denote ORC and a plus denotes a licensed origin. The metaphase chromosome decondenses, assembles licensed origins, is assembled into a nucleus, replicates, and then recondenses. Below are the activities of the origin recognition system, the licensing system and SPF during the course of the cell cycle. See text for further details.
None
Fig. 2. Events occurring at a replication origin in the Xenopus early embryo from late mitosis to late G1. (A) Assembly of ORC onto origin DNA. (B) Binding of Cdc6 and RLF-B/Cdt1 onto ORC. (C) Multiple Mcm(2–7) heterohexamers are assembled onto each origin to license it. (D) Once licensing is complete Cdc6 is removed, whilst ORC binds less tightly (unshaded ORC denotes weak binding to DNA). (E) Cdc7 is recruited to the licensed origin and phosphorylates Mcm(2–7) complexes (purple circles denote phosphorylation). (F) Following nuclear assembly, CDK activity induces the assembly of Cdc45 onto the origin.
See this image and copyright information in PMC

References

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    1. Blow J.J. and Laskey,R.A. (1988) A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature, 332, 546–548. - PubMed
    1. Blow J.J., Gillespie,P.J., Francis,D. and Jackson,D.A. (2001) Replication origins in Xenopus egg extract are 5–15 kb apart and are activated in clusters that fire at different times. J. Cell Biol., 152, 15–26. - PMC - PubMed
    1. Blumenthal A.B., Kriegstein,H.J. and Hogness,D.S. (1974) The units of DNA replication in Drosophila melanogaster chromosomes. Cold Spring Harb. Symp. Quant. Biol., 38, 205–223. - PubMed
    1. Carpenter P.B., Mueller,P.R. and Dunphy,W.G. (1996) Role for a Xenopus Orc2-related protein in controlling DNA replication. Nature, 379, 357–360. - PubMed

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