Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Jul;3(7):613-8.
doi: 10.1038/35083000.

Phox domain interaction with PtdIns(3)P targets the Vam7 t-SNARE to vacuole membranes

M L Cheever  1 , T K Sato, T de Beer, T G Kutateladze, S D Emr, M Overduin
Affiliations

Phox domain interaction with PtdIns(3)P targets the Vam7 t-SNARE to vacuole membranes

M L Cheever et al. Nat Cell Biol. 2001 Jul.

Abstract

Specific recognition of phosphoinositides is crucial for protein sorting and membrane trafficking. Protein transport to the yeast vacuole depends on the Vam7 t-SNARE and its phox homology (PX) domain. Here, we show that the PX domain of Vam7 targets to vacuoles in vivo in a manner dependent on phosphatidylinositol 3-phosphate generation. A novel phosphatidylinositol-3-phosphate-binding motif and an exposed loop that interacts with the lipid bilayer are identified by nuclear magnetic resonance spectroscopy. Conservation of key structural and binding site residues across the diverse PX family indicates a shared fold and phosphoinositide recognition function.

PubMed Disclaimer

Comment in

Publication types

MeSH terms