Power spectral analysis imperfectly informs changes in sympathetic traffic during acute simulated microgravity

Abstract

The purpose of this study was to investigate the value of frequency-domain analysis of autonomic rhythms as a simple, non-invasive technique for the study of immediate neural adjustments to simulated microgravity. We continuously recorded the electrocardiogram, non-invasive beat-by-beat arterial pressure, and muscle sympathetic nerve activity (MSNA) during 5-min periods of controlled frequency breathing (15 breaths.min-1) with subjects (n = 10) in supine, and 10 degrees head-down tilt positions. We estimated changes in fluid volume with lower leg circumference measurements. We analyzed data in the frequency domain with fast Fourier-based power spectral analysis, and calculated the ratio of normalized low-to-respiratory frequency RR-interval spectral power as an index of sympathetic activity. Head-down tilt significantly reduced lower leg volume, MSNA, and MSNA oscillations at the respiratory frequency (p < 0.05). Head-down tilt did not change RR-interval, arterial pressure, or their power spectra (p > 0.05). We conclude that non-invasive frequency-domain estimates do not adequately reveal subtle changes in sympathetic traffic during acute, simulated microgravity.