Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 May;65(5):1175-80.
doi: 10.1271/bbb.65.1175.

Cloning and characterization of the cpyA gene encoding intracellular carboxypeptidase from Aspergillus nidulans

K Ohsumi  1 , Y Matsuda, H Nakajima, K Kitamoto
Affiliations
Free article

Cloning and characterization of the cpyA gene encoding intracellular carboxypeptidase from Aspergillus nidulans

K Ohsumi et al. Biosci Biotechnol Biochem. 2001 May.
Free article

Abstract

Carboxypeptidase Y (CPY) has been used as a maker enzyme for investigations on intracellular transport of vacuolar proteins and on vacuolar biogenesis in Saccharomyces cerevisiae. We describe the cloning and characterization of the CPY homologue encoding gene (cpyA) from the filamentous fungus Aspergillus nidulans. The cpyA gene has one intron and encodes a protein with 552 amino acids containing a putative signal sequence and pro-sequence. The predicted CpyA protein is highly similar in sequence with carboxypeptidases from several yeast species and contains a catalytic triad (Asp-His-Ser) like that of serine carboxypeptidase. The cpyA disruptant cells showed reduced levels of intracellular carboxypeptidase. These results suggest that the cpyA gene encodes a vacuolar carboxypeptidase in A. nidulans.

PubMed Disclaimer