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. 2001 May;46(2):161-70.
doi: 10.1023/a:1010679901911.

Characterization of an ATP-dependent type I DNA ligase from Arabidopsis thaliana

Y Q Wu  1 , B Hohn, A Ziemienowic
Affiliations

Characterization of an ATP-dependent type I DNA ligase from Arabidopsis thaliana

Y Q Wu et al. Plant Mol Biol. 2001 May.

Abstract

Here we report the purification and biochemical characterization of recombinant Arabidopsis thaliana DNA ligase I. We show that this ligase requires ATP as a source for adenylation. The calculated Km [ATP] for ligation is 3 microM. This enzyme is able to ligate nicks in oligo(dT)/poly(dA) and oligo(rA)/poly(dT) substrates, but not in oligo(dT)/poly(rA) substrates. Double-stranded DNAs with cohesive or blunt ends are also good substrates for the ligase. These biochemical features of the purified enzyme show the characteristics typical of a type I DNA ligase. Furthermore, this DNA ligase is able to perform the reverse reaction (relaxation of supercoiled DNA) in an AMP-dependent and PPi-stimulated manner.

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