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Comparative Study
. 2001 Aug;45(8):2386-9.
doi: 10.1128/AAC.45.8.2386-2389.2001.

Cloning and biochemical characterization of a class A beta-lactamase from Prevotella intermedia

I Madinier  1 , T Fosse, J Giudicelli, R Labia
Affiliations
Comparative Study

Cloning and biochemical characterization of a class A beta-lactamase from Prevotella intermedia

I Madinier et al. Antimicrob Agents Chemother. 2001 Aug.

Abstract

The gene encoding a beta-lactamase of Prevotella intermedia was cloned and sequenced. This gene, called cfxA2, shared 98% identity with cfxA, the structural gene of a beta-lactamase previously described in Bacteroides vulgatus. The deduced protein sequence had a K272E substitution. CfxA2 had the characteristics of class A, group 2e beta-lactamases.

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FIG. 1
FIG. 1
Multiple sequence alignment of the amino acid sequences of CfxA2 and related β-lactamases. Amino acids that are identical in all five sequences are marked with asterisks. The amino acid numbering for the class A β-lactamases is used (2). The main conserved amino acid motifs of class A β-lactamases are shaded. Abbreviations: P.i, P. intermedia NI-1187; B.fr, B. fragilis (17); S. ty, S. enterica serovar Typhimurium (4); P. ae, P. aeruginosa (14); B.un, B. uniformis (19). CfxA2 differs from CfxA of B. vulgatus by a K272E substitution (16).
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