[Microtiter agglutination test for salmonella and brucella antibodies (author's transl)]

Abstract

To compare the tube agglutination test and the microtiter plate agglutination procedure, 207 human sera were tested for agglutinins to Salmonella and 267 sera for agglutinins to Brucella by both methods. A positive reaction in the microtiter test was indicated by a smooth or irregular mat shape at the bottom of each well in the microtiter plate, whereas a negative reaction was shown by a settling of the antigen to a button shape. As can be seen in table 1, identical titer or only one dilution difference between both tests were found in more than 93% of the samples tested. The plate test was easier to perform and saved time, space, antigen and serum. The microtiter-agglutination technique can, therefore, be recommended for the routine laboratory for serology.