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. 2001 Aug;39(8):2987-90.
doi: 10.1128/JCM.39.8.2987-2990.2001.

Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis clinical isolates from India

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Mutations in the rpoB gene of multidrug-resistant Mycobacterium tuberculosis clinical isolates from India

C Mani et al. J Clin Microbiol. 2001 Aug.

Abstract

Mutations in the 81-bp rifampin resistance-determining region (RRDR) of the rpoB gene were analyzed by DNA sequencing of 50 Mycobacterium tuberculosis clinical isolates (44 resistant and 6 sensitive) from various parts of India. Fifty-three mutations of 18 different kinds, 17 point mutations and one deletion, were observed in 43 of 44 resistant isolates. Three novel mutations and three new alleles within the RRDR, along with two novel mutations outside the RRDR, are reported in this study.

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Figures

FIG. 1
FIG. 1
Mutations in the RRDR of the rpoB gene of M. tuberculosis Indian isolates. The bottom panel shows the mutated codons with corresponding amino acids. The original sequence is shown boxed. Numbers to the left of or below amino acid designations indicate numbers of isolates showing the mutation, while percentages denote the frequencies of occurrence of mutations at the particular codon.

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