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. 2001 Aug;39(8):2991-4.
doi: 10.1128/JCM.39.8.2991-2994.2001.

Possible approach for serodiagnosis of ascariasis by evaluation of immunoglobulin G4 response using Ascaris lumbricoides somatic antigen

T Bhattacharyya  1 , A Santra, D N Majumder, B P Chatterjee
Affiliations

Possible approach for serodiagnosis of ascariasis by evaluation of immunoglobulin G4 response using Ascaris lumbricoides somatic antigen

T Bhattacharyya et al. J Clin Microbiol. 2001 Aug.

Abstract

Somatic antigen of Ascaris lumbricoides was purified to homogeneity (molecular mass, 34 kDa) by ammonium sulfate fractionation and successive chromatographic procedures, namely, gel permeation, ion exchange, and high-performance gel permeation liquid chromatographies. The antigen showed strong binding with immunoglobulin G (IgG) in Ascaris-infested patients and was cross-reactive with IgE and IgG in patients infested with other nematodes. It reacted specifically with IgG4 (P < 0.001) in 63 Ascaris-infested patients, which represented 65% of the total IgG response, though cross-reactivity with IgG1, IgG2, and IgG3 subclasses was observed, indicating the unique specificity of this test system and its potential utility in the serodiagnosis of ascariasis.

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Figures

FIG. 1
FIG. 1
(a) Gel permeation chromatography of the 30 to 70% ammonium sulfate fraction of A. lumbricoides SAg on a Sephacryl S-300 column. (b) Separation of Als III on a Resource-Q anion exchanger by FPLC. (c) Purification of Als IIIb by HPGPLC on a protein PAC 300 SW column. Abs., absorbance.
FIG. 2
FIG. 2
pSAg-specific IgE and IgG response in patients with different nematode infections. ←, control cut-off value for IgG; →, control cut-off value for IgE. Dark gray bars, specific IgE response; light gray bars, specific IgG response. A, A. lumbricoides; H, hookworm; T, T. trichura; S, S. stercoralis; C, control. Abs., absorbance.
FIG. 3
FIG. 3
pSAg-specific IgG4 response by ELISA in sera of different groups of individuals: Ascaris-infested (n = 63), hookworm-infested (n = 10), T. trichura-infested (n = 10), S. stercoralis-infested (n = 10), and control (n = 10) subjects.
FIG. 4
FIG. 4
Relationship between absorbance levels of IgG4 response and A. lumbricoides worm load. Abs., absorbance.
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