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Comparative Study
. 2001 Aug;39(8):3006-8.
doi: 10.1128/JCM.39.8.3006-3008.2001.

Rapid pulsed-field gel electrophoresis method for group B streptococcus isolates

J A Benson  1 , P Ferrieri
Affiliations
Comparative Study

Rapid pulsed-field gel electrophoresis method for group B streptococcus isolates

J A Benson et al. J Clin Microbiol. 2001 Aug.

Abstract

We developed a rapid pulsed-field gel electrophoresis (PFGE) method that required 3 days to complete, an improvement over the standard method that required as many as 8 days. The accuracy and reproducibility of the rapid method were verified by analysis of DNA band sizes of our control group B streptococcus isolate. The rapid method was superior to the standard method, providing more precise molecular sizing and gels of higher image quality. The reproducibility of rapid PFGE substantiated its value and continued use.

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Figures

FIG. 1
FIG. 1
PFGE gel from the standard method and the rapid method stained with ethidium bromide. The standard-method gel (left) was run for 29 h at 10°C, and the rapid-method gel (right) was run for 22 h at 14°C. Both gels were run at 6.0 V/cm and used switching times of 10 to 45 s. Lane 1, lambda ladder. Lanes 2 to 8, GBS type V/R1, R4 isolates. Lane 9, control GBS strain 89-022 (type Ib/α+β).
See this image and copyright information in PMC

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