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Review
. 2001 Jun;5(3):314-24.
doi: 10.1016/s1367-5931(00)00208-8.

Random-peptide libraries and antigen-fragment libraries for epitope mapping and the development of vaccines and diagnostics

M B Irving  1 , O Pan, J K Scott
Affiliations
Review

Random-peptide libraries and antigen-fragment libraries for epitope mapping and the development of vaccines and diagnostics

M B Irving et al. Curr Opin Chem Biol. 2001 Jun.

Abstract

Random peptide libraries and antigen-fragment libraries (also known as gene-fragment libraries) have been used to identify epitopes on protein antigens. These technologies promise to make significant contributions to diagnostic and vaccine development. Researchers in a number of labs have shown that phage selected from libraries with protective antibodies, raised against whole antigen, can be used as immunogens to stimulate antibody responses that bind native antigen and provide protection in vivo. Others have used the sera of patients with idiopathic diseases to screen libraries, and by this approach have identified candidate antigens involved in immune disease. These may prove useful for diagnosis and, possibly, in determining disease etiology.

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Figures

Fig. 1
Fig. 1
The filamentous phage for recombinant display of foreign peptide or protein. (a) Schematic of wild-type Ff phage and the major (pVIII) and minor (pIII, PVI, pVII, PIX) coat proteins. A comparison of full and hybrid display of foreign peptides or proteins via (b) pIII and (c) pVIII.The filamentous phage for recombinant display of foreign peptide or protein. (a) Schematic of wild-type Ff phage and the major (pVIII) and minor (pIII, PVI, pVII, PIX) coat proteins. A comparison of full and hybrid display of foreign peptides or proteins via (b) pIII and (c) pVIII.
See this image and copyright information in PMC

References

    1. Geysen H.M, Tainer J.A, Rodda S.J, Mason T.J, Alexander H, Getzoff E.D, Lerner R.A. Chemistry of antibody binding to a protein. Science. 1987;235:1184–1190. - PubMed
    1. Davies D.R, Cohen G.H. Interactions of protein antigens with antibodies. Proc Natl Acad Sci USA. 1996;93:7–12. - PMC - PubMed
    1. Barlow D.J, Edwards M.S, Thornton J.M. Continuous and discontinuous protein antigenic determinants. Nature. 1986;322:747–748. - PubMed
    1. Getzoff E.D, Geysen H.M, Rodda S.J, Alexander H, Tainer J.A, Lerner R.A. Mechanisms of antibody binding to a protein. Science. 1987;235:1191–1196. - PubMed
    1. Smith G.P. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science. 1985;228:1315–1317. - PubMed