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. 1975 Mar;146(3):741-3.
doi: 10.1042/bj1460741.

A micro method for simultaneous determination of galactosyltransferase and 5'-nucleotidase activities in cell fractions

L S Freilich, M E Richmond, A C Reppucci Jr, J E Silbert

A micro method for simultaneous determination of galactosyltransferase and 5'-nucleotidase activities in cell fractions

L S Freilich et al. Biochem J. 1975 Mar.

Abstract

Galactosyltransferase and 5'-nucleotidase were assayed in the same reaction mixture, with ovalbumin as exogenous acceptor of (14-C)galactose and with (3-H)AMP as the substrate for the 5'-nucleotidase assay. The substrates and reaction products of either assay had no significant effect on the activity of the other enzyme.

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