doi: 10.1038/90802.
Cell-free translation reconstituted with purified components
Affiliations
- PMID: 11479568
- DOI: 10.1038/90802
Item in Clipboard
Cell-free translation reconstituted with purified components
Nat Biotechnol.
2001 Aug.
Abstract
We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 microg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).
Comment in
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A PURE approach to constructive biology.Nat Biotechnol. 2001 Aug;19(8):732-3. doi: 10.1038/90773. Nat Biotechnol. 2001. PMID: 11479561 No abstract available.
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