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. 2001 Aug;19(8):751-5.
doi: 10.1038/90802.

Cell-free translation reconstituted with purified components

Y Shimizu  1 , A Inoue, Y Tomari, T Suzuki, T Yokogawa, K Nishikawa, T Ueda
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Cell-free translation reconstituted with purified components

Y Shimizu et al. Nat Biotechnol. 2001 Aug.

Abstract

We have developed a protein-synthesizing system reconstituted from recombinant tagged protein factors purified to homogeneity. The system was able to produce protein at a rate of about 160 microg/ml/h in a batch mode without the need for any supplementary apparatus. The protein products were easily purified within 1 h using affinity chromatography to remove the tagged protein factors. Moreover, omission of a release factor allowed efficient incorporation of an unnatural amino acid using suppressor transfer RNA (tRNA).

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