Quantitative regulation of the Flaveria Me1 gene is controlled by the 3'-untranslated region and sequences near the amino terminus

Abstract

The Me1 gene of Flaveria bidentis codes for the C4 isoform of NADP malic enzyme, which accumulates to a high-level only in bundle sheath cells. Previous experiments demonstrated that sequences at the 5' end of the gene control cell specificity whereas sequences at the 3' end are necessary for high-level expression. To localize quantitative regulator sequences, we have analysed a series of Me1 3' deletion constructs fused to the gusA reporter gene. We show that sequences within the 3'-untranslated region (3'-UTR) control quantitative levels of expression. Analysis of 5' promoter fusions demonstrated that high-level expression also requires sequences within the N-terminal coding region of the gene, suggesting possible interactions between the 3'-UTR and 5' coding regions. Cell-specific regulatory sequences are located in a different part of the 5' end of the gene, between 1023 bp upstream of the transcription start and the start of translation.