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. 1975 Jun 18;17(4):263-71.
doi: 10.1007/BF02546599.

The composition of the insoluble collagenous matrix of bovine predentine

D J Carmichael, A Chovelon, C H Pearson

The composition of the insoluble collagenous matrix of bovine predentine

D J Carmichael et al. Calcif Tissue Res. .

Abstract

Predentine obtained from bovine teeth by microdissection was extracted with EDTA and tris-NaCl solutions. The insoluble residue consisted mainly of collagen and resembled dentine collagen in overall amino acid composition. The residue differed in containing no detectable phosphoprotein, a much larger amount of collagen hexose and more non-collagenous glycoprotein, the neutral sugar composition of which was determined. Differences were also observed in the contents of reducible collagen cross-links. Half of the total phosphorus found in the predentine could not be accounted for solely by hydroxyapatite. The remainder was partly soluble and dialysable.

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