doi: 10.1007/BF02546233.
A quantitative method for the application of compressive forces to bone in tissue culture
- PMID: 1148895
- DOI: 10.1007/BF02546233
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A quantitative method for the application of compressive forces to bone in tissue culture
Calcif Tissue Res.
.
Abstract
A quantitative method for the application of compressive forces to long bones in culture is described. Using this method it was found that a physiological pressure of 80g/cm sq. applied to tibiae of 16-day-old chick embryos reduced glucose consumption to 50% of controls. Twenty four hours after the release of pressure glucose utilization again increased, approaching control levels. A pressure of 80 g/cm sq. also stimulated thymidine incorporation into DNA. The same pressure decreased the size of the extracellular fluid pool by 8%, but had no effect on the rate of equilibration of this pool with 22Na.
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