[Direct determination of inorganic phosphor in serum and urine utilizing Rhodamin B (author's transl)]

Abstract

A micromethod is described for the spectrophotometric determination of inorganic phosphate in serum and urine utilizing Rhodamin B as a dye (phosphomolybdat-Rhodamin B complex), Brij 35, and polyvinylpyrrolidon as catalyst. The procedure does not involve deproteinization and yields a stable complex in 20 min. The linearity is constant up to at least 9 mg Pi per 100 ml. It is both accurate (r = 0.972 in serum, r = 0.989 in urine, recovery in urine 98%) and precise (vk = 1.16% in the series). Bilirubin up to 10 mg/100 ml and serum protein do not interfere with the method.