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Clinical Trial
. 2001 Jun;18(6):325-9.
doi: 10.1023/a:1016632621452.

Pregnancies resulting from in vitro matured oocytes collected from women with regular menstrual cycle

H G Yoon  1 , S H Yoon, W Y Son, S W Lee, S P Park, K S Im, J H Lim
Affiliations
Clinical Trial

Pregnancies resulting from in vitro matured oocytes collected from women with regular menstrual cycle

H G Yoon et al. J Assist Reprod Genet. 2001 Jun.

Abstract

Purpose: To demonstrate that human immature oocytes retrieved from women with regular menstrual cycles can undergo maturation and fertilization, and that the resulting embryos can establish pregnancies.

Methods: Immature oocytes (n = 568) were retrieved from women with regular menstrual cycle. The intact immature oocytes (n = 506) were allowed to mature in YS medium supplemented with 70% human follicular fluid (hFF); the matured oocytes were fertilized with husband sperm. Two pronuclei oocytes were cocultured with cumulus cells in YS medium supplemented with 10% hFF until 2 or 3 days after insemination. The cleaved embryos were transferred in uteri.

Results: Follicles were aspirated on Day 9.2 +/- 5.3 of 63 natural cycles from 51 patients (mean age = 34.8 +/- 4.0 years). The average number of retrieved immature oocytes was 9.0. The maturation rate was 74.3% (376/506). The two PN and cleavage rates were 72.6% (273/376) and 89.0% (243/273), respectively. Embryo transfer was achieved in 51 cycles and clinical pregnancy rate was 17.6% (9/51).

Conclusions: The results suggest that in vitro matured oocytes can undergo fertilization and the resulting embryos may successfully lead to pregnancies. However, further research is needed to improve IVM technique to achieve success rate comparable to gonadotrophin stimulated cycles.

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References

    1. Roest J, Mous HV, Zeilmaker GH, Verhoeff A. The incidence of major clinical complications in a Dutch transport IVF program. Hum Reprod Update. 1996;2:345–353. - PubMed
    1. Steward JA, Hamilton PJ, Murdoch AP. Thromboembolic disease associated with ovarian stimulation and assisted conception techniques. Hum Reprod. 1997;12:2167–2173. - PubMed
    1. Whittemore AS, Harris R, Itnyre J, Collaborative Ovarian Cancer Group Characteristics relating to ovarian cancer risk: Collaborative analysis of 12 US case-control studies. Am J Epidemiol. 1992;136:1184–1203. - PubMed
    1. Veeck LL, Wortham JW, Jr, Witmyer J. Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril. 1983;39:594–602. - PubMed
    1. Nagy ZP, Cecile J, Liu J, Loccufier A, Devroey P, Van Steirteghem A. Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: Case report. Fertil Steril. 1996;65:1047–1050. - PubMed

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