Accumulation of basic drugs in 5-hydroxytryptamine storage organelles of rabbit blood platelets

Abstract

The subcellular distribution of basic substances such as mepacrine, chloroquine, chlorpromazine and imipramine was studied in rabbit blood platelets exposed to these compounds in vitro and to some in vivo. All the drugs showed a preferential concentration in the 5-hydroxytryptamine (5HT) storage organelles (5HT vesicles). Chloroquine and mepacrine accumulated more specifically in the organelles than did chlorpromazine and imipramine. After osmotic shock of the 5HT vesicles chlorpromazine sedimented mostly with the vesicular membranes, whereas the other compounds preferentially went into the supernatant. It is concluded that (a) fluorescent basic compounds such as mepacrine are rather specific markers for the 5HT storage organelles in live platelets and (b) drugs such as chlorpromazine and imipramine also have a considerable affinity for these organelles.