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. 2001;151(1):29-33.
doi: 10.1023/a:1010960402254.

Isolation of aflatoxigenic strains of Aspergillus and detection of aflatoxin B1 from feeds in India

T K Dutta  1 , P Das
Affiliations

Isolation of aflatoxigenic strains of Aspergillus and detection of aflatoxin B1 from feeds in India

T K Dutta et al. Mycopathologia. 2001.

Abstract

In a preliminary study, 256 feed samples collected from different parts of Northern India were examined for the presence of aflatoxigenic strains of Aspergillus favus/parasiticus and for detection of Aflatoxin B1 (AFB1). Out of 198 A. flavus and 15 A. parasiticus strains isolated, 76% and 86% respectively, were found to be toxigenic. Aflatoxin B1 content of these feeds, as estimated by thin layer chromatography (TLC) and enzyme linked immunosorbent assay (ELISA) were very high (average 0.412+/-0.154 ppm) in comparison to the permissible Indian regulation level (0.03 ppm). Seasonal variation of incidence and level of toxin in feed was recorded and it was high during monsoon/post monsoon period.

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