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. 1975 Feb 26;105(2):323-8.
doi: 10.1016/s0021-9673(01)82261-5.

Quantitation of tryptophan metabolites in rat feces by thin-layer chromatography

G M Anderson

Quantitation of tryptophan metabolites in rat feces by thin-layer chromatography

G M Anderson. J Chromatogr. .

Abstract

Indole, skatole, indole-3-acetic acid, indole-3-propionic acid, tryptamine, and free tryptophan have been extracted from rat fecal samples and quantitated by thin-layer chromatography. Additional tryptophan metabolites have been recovered in yields of 87-97% from supplemented fecal samples. Detection limits for all the compounds studied were in the 0.1 mug/g feces range. Quantitation was possible in the mug/g range with an estimated accuracy of plus or minus 10%.

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