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. 1975 Mar 19;106(1):83-95.
doi: 10.1016/s0021-9673(01)81049-9.

Analysis of insect pheromones by quadrupole mass fragmentography and high-resolution gas chromatography

H R Buser, H Arn

Analysis of insect pheromones by quadrupole mass fragmentography and high-resolution gas chromatography

H R Buser et al. J Chromatogr. .

Abstract

A sensitive technique is described for the analysis of insect pheromones. Insect extracts are separated into fractions by column chromatography and are analyzed directly by quadrupole mass fragmentography on high-resolution glass capillary columns and by mass spectrometry. Applications are illustrated with two important pests. In the codling moth, trans-8, trans-10-dodecadien-1-ol was found to be present at a level of 3.5 ng per female, and in the European grapevine moth trans-7, cis-9-dodecadienyl acetate at a level of 1.6 ng. Both compounds appear to be present in high isomeric purity in the female insect and are absent in the corresponding males.

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