doi: 10.1016/s0378-4347(01)00260-2.
Simple and rapid high-performance liquid chromatography method for the determination of ofloxacin concentrations in plasma and urine
Affiliations
- PMID: 11522070
- DOI: 10.1016/s0378-4347(01)00260-2
Item in Clipboard
Simple and rapid high-performance liquid chromatography method for the determination of ofloxacin concentrations in plasma and urine
J Chromatogr B Biomed Sci Appl.
.
Abstract
A high-performance liquid chromatographic method for the determination of ofloxacin in human plasma and urine was developed. The method involved deproteinisation of the sample with perchloric acid and analysis of the supernatant using a reversed-phase C18 column and fluorescence detection at an excitation wavelength of 290 nm and an emission wavelength of 460 nm. The assay was linear from 0.5 to 10.0 microg/ml. The relative standard deviation of intra- and inter-day assays was lower than 5%. The average recovery of ofloxacin from plasma was 93%. The method was evaluated in samples from healthy subjects whose drug levels were already measured by microbiological assay.
Similar articles
-
Rapid stereospecific high-performance liquid chromatographic determination of levofloxacin in human plasma and urine.J Pharm Biomed Anal. 1997 Mar;15(6):765-71. doi: 10.1016/s0731-7085(96)01890-0. J Pharm Biomed Anal. 1997. PMID: 9172102
-
Determination of ofloxacin in human plasma using high-performance liquid chromatography and fluorescence detection.J Chromatogr B Biomed Appl. 1995 Nov 17;673(2):316-9. doi: 10.1016/0378-4347(95)00263-5. J Chromatogr B Biomed Appl. 1995. PMID: 8611968
-
Determination of the antibacterial ofloxacin in human urine and serum samples by solid-phase spectrofluorimetry.J Pharm Biomed Anal. 2002 Nov 7;30(4):1103-10. doi: 10.1016/s0731-7085(02)00466-1. J Pharm Biomed Anal. 2002. PMID: 12408901
-
Determination of norfloxacin in human plasma and urine by high-performance liquid chromatography and fluorescence detection.J Chromatogr A. 1998 Jul 3;812(1-2):381-5. doi: 10.1016/s0021-9673(98)00401-4. J Chromatogr A. 1998. PMID: 9691333 Clinical Trial.
-
Determination of levofloxacin in plasma, bronchoalveolar lavage and bone tissues by high-performance liquid chromatography with ultraviolet detection using a fully automated extraction method.J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jan 5;799(1):165-72. doi: 10.1016/j.jchromb.2003.10.031. J Chromatogr B Analyt Technol Biomed Life Sci. 2004. PMID: 14659449
Cited by
-
validated microbiological and HPLC methods for the determination of moxifloxacin in pharmaceutical preparations and human plasma.Braz J Microbiol. 2012 Oct;43(4):1291-301. doi: 10.1590/S1517-83822012000400008. Epub 2012 Jun 1. Braz J Microbiol. 2012. PMID: 24031955 Free PMC article.
-
Simultaneous RPHPLC Determination of Nitazoxanide and Ofloxacin in Combined Tablet Dosage Form.Indian J Pharm Sci. 2008 Jul-Aug;70(4):491-4. doi: 10.4103/0250-474X.44600. Indian J Pharm Sci. 2008. PMID: 20046777 Free PMC article.
-
Simultaneous spectrophotometric estimation of nitazoxanide and ofloxacin in tablets.Indian J Pharm Sci. 2011 Jan;73(1):70-4. doi: 10.4103/0250-474X.89759. Indian J Pharm Sci. 2011. PMID: 22131624 Free PMC article.