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. 2001 Sep;67(9):4382-4.
doi: 10.1128/AEM.67.9.4382-4384.2001.

Rapid confirmation of Clostridium perfringens by using chromogenic and fluorogenic substrates

P W Adcock  1 , C P Saint
Affiliations

Rapid confirmation of Clostridium perfringens by using chromogenic and fluorogenic substrates

P W Adcock et al. Appl Environ Microbiol. 2001 Sep.

Abstract

The use of 4-methylumbelliferyl phosphate (MUP) and ortho-nitrophenyl-beta-D-galactopyranoside (ONPG) for the identification of Clostridium perfringens was investigated. A liquid assay containing both MUP and ONPG was a highly specific alternative method for C. perfringens confirmation, reducing incubation time from 48 to only 4 h. The assay solution is easy to prepare, does not require anaerobic conditions for use, and has an extended shelf life.

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References

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