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. 2001 Sep;39(9):3350-2.
doi: 10.1128/JCM.39.9.3350-3352.2001.

Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis

K J Edwards  1 , L A Metherell, M Yates, N A Saunders
Affiliations

Detection of rpoB mutations in Mycobacterium tuberculosis by biprobe analysis

K J Edwards et al. J Clin Microbiol. 2001 Sep.

Abstract

A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.

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Figures

FIG. 1
FIG. 1
Biprobe peaks observed with M. tuberculosis strains that have mutations at codons 511 (A) 516 (B), and 526 and 531 (C). −dF/dT is the first negative derivative of the change in fluorescence (dF) divided by the change in temperature (dT).
See this image and copyright information in PMC

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References

    1. Bio/Gene Ltd. and the Secretary of State for Defense. July 1999. Nucleic acid detection system. British patent GB2333359A.
    1. Brown T J, Tansel O, French G L. Simultaneous identification and typing of multi-drug-resistant Mycobacterium tuberculosis isolates by analysis of pncA and rpoB. J Med Microbiol. 2000;49:651–656. - PubMed
    1. Caugant D A, Sandven P, Eng J, Jeque J T, Tonjum T. Detection of rifampicin resistance among isolates of Mycobacterium tuberculosis from Mozambique. Microb Drug Resist. 1995;1:321–326. - PubMed
    1. De Beenhouwer H, Lhiang Z, Jannes G, Mijs W, Machtelinckx L, Rossau R, Traore H, Portaels F. Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay. Tuber Lung Dis. 1995;76:425–430. - PubMed
    1. Joint Tuberculosis Committee of the British Thoracic Society. Chemotherapy and management of tuberculosis in the United Kingdom: recommendations. Thorax. 1998;53:536–548. - PMC - PubMed

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