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. 2001 Sep;39(9):3353-5.
doi: 10.1128/JCM.39.9.3353-3355.2001.

Existence of hepatitis C virus in Culex quinquefasciatus after ingestion of infected blood: experimental approach to evaluating transmission by mosquitoes

T T Chang  1 , T Y Chang, C C Chen, K C Young, J N Roan, Y C Lee, P N Cheng, H L Wu
Affiliations

Existence of hepatitis C virus in Culex quinquefasciatus after ingestion of infected blood: experimental approach to evaluating transmission by mosquitoes

T T Chang et al. J Clin Microbiol. 2001 Sep.

Abstract

We used PCR to detect hepatitis C virus (HCV) RNA among supernatants of ground Culex quinquefasciatus mosquitoes that (i) had been fed HCV-positive blood, (ii) had been intrathoracically inoculated with HCV-positive blood, or (iii) were from homes of hepatitis C patients. HCV RNA was detectable under all three conditions, but it did not replicate in mosquitoes and was not detectably transmitted during feeding.

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Figures

FIG. 1
FIG. 1
Nucleotide sequence alignment of the 5′ noncoding region, core, E1/E2, NS3, and NS5 fragments of HCV from a patient and mosquitoes. The sequences are compared with the Taiwanese strain of HCV, as published by Chen et al. (2) (EMBL/GenBank M84754). The nucleotide numbering system is according to that of GenBank sequence number M84754.
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References

    1. Chang T T, Young K C, Yang Y J, Lai K A, Wu H L, Wu M H, Chen M Y, Lin X Z, Lin C Y, Shin J S. Incidence of post-transfusion hepatitis in Taiwan before and after introduction of anti-HCV testing. Liver. 1996;16:201–206. - PubMed
    1. Chen P J, Lin M H, Tai K F, Liu P C, Lin C J, Chen D S. The Taiwanese hepatitis C virus genome: sequence determination and mapping the 5′termini of viral genomic and antigenomic RNA. Virology. 1992;188:102–113. - PubMed
    1. Fouche A, Crewe R M, Windsor I M, Karim S S. Persistence of hepatitis B antigen in Culex quinquefasciatus (Diptera: Culicidae) J Med Entomol. 1994;27:697–700. - PubMed
    1. Rosen L, Gubler D. The use of mosquitoes to detect and propagate dengue virus. Am J Trop Med Hyg. 1974;23:1153–1160. - PubMed
    1. Schoepp R J, Beaty B J, Eckels K H. Dengue 3 virus infection of Aedes albopictus and Aedes aegypti: comparison of parent and progeny candidate vaccine viruses. Am J Trop Med Hyg. 1990;42:89–96. - PubMed

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