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. 2001 Sep;39(9):3382-5.
doi: 10.1128/JCM.39.9.3382-3385.2001.

Application of PCR to distinguish common species of dermatophytes

E Faggi  1 , G Pini, E Campisi, C Bertellini, E Difonzo, F Mancianti
Affiliations

Application of PCR to distinguish common species of dermatophytes

E Faggi et al. J Clin Microbiol. 2001 Sep.

Abstract

This report describes the application of PCR fingerprinting for the identification of species and varieties of common dermatophytes and related fungi utilizing as a single primer the simple repetitive oligonucleotide (GACA)(4). The primer was able to amplify all the strains, producing species-specific profiles for Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Trichophyton ajelloi, and Epidermophyton floccosum. Intraspecific variability was not observed for these species. Instead, three different profiles were observed in the Trichophyton mentagrophytes group.

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Figures

FIG. 1
FIG. 1
PCR fingerprints of M. canis strains (each strain was amplified in duplicate). Lanes: M, molecular weight marker VI (Boehringer Mannheim), size range, 154 to 2,176 bp; 1, control reaction without template DNA; 2 to 7, three strains from humans; 8 to 15, four strains from dogs; 16 to 19, two strains from cats.
FIG. 2
FIG. 2
PCR fingerprints of five different dermatophyte species (two different strains from each species). Lanes: M, molecular weight marker VI (Boehringer Mannheim), size range, 154 to 2,176 bp; 1 and 2, M. canis; 3 and 4, M. gypseum; 5 and 6, T. rubrum; 7 and 8, T. ajelloi; 9 and 10, E. floccosum.
FIG. 3
FIG. 3
PCR fingerprints showing three different profiles of T. mentagrophytes group strains (each strain amplified in duplicate). Lanes: M, molecular weight marker VI (Boehringer Mannheim), size range, 154 to 2,176 bp; 1 and 2, T. mentagrophytes var. granulosum; 3 to 6, T. interdigitale (two strains); 7 and 8, T. mentagrophytes var. asteroides; 9 and 10, T. mentagrophytes var. radians; 11 and 12, T. mentagrophytes var. lacticolor; 13 and 14, T. mentagrophytes of unspecified variety.
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