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. 2001 Sep;8(5):1018-20.
doi: 10.1128/CDLI.8.5.1018-1020.2001.

Determination of human immunodeficiency virus type 1 subtypes by a rapid method useful for the routine diagnostic laboratory

H H Kessler  1 , D Deuretzbacher, E Stelzl, E Daghofer, B I Santner, E Marth
Affiliations

Determination of human immunodeficiency virus type 1 subtypes by a rapid method useful for the routine diagnostic laboratory

H H Kessler et al. Clin Diagn Lab Immunol. 2001 Sep.

Abstract

The existence of human immunodeficiency virus type 1 (HIV-1) subtypes has many important implications for the global evolution of HIV and for the evaluation of pathogenicity, transmissibility, and candidate HIV vaccines. The aim of this study was to establish a rapid method for determination of HIV-1 subtypes useful for a routine diagnostic laboratory and to investigate the distribution of HIV-1 subtypes in Austrian patients. Samples were tested by a subtyping method based on a 1.3-kb sequence of the polymerase gene generated by a commercially available drug resistance assay. The generated sequence was subtyped by means of an HIV sequence database. Results of 74 routine samples revealed subtype B (71.6%) as the predominant subtype, followed by subtype A (13.5%) and subtype C (6.8%). Subtypes E, F, G, and AE (CM240) were also detected. This subtyping method was found to be very easy to handle, rapid, and inexpensive and has proved suitable for high-throughput routine diagnostic laboratories. The specific polymerase gene sequence, however, must be existent.

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Figures

FIG. 1
FIG. 1
Prevalence of HIV-1 subtypes in Austrian HIV-1 patients.
See this image and copyright information in PMC

References

    1. Adwan G, Papa A, Kouidou S, Alexiou S, Ialissiovas N, Itoutsos I, Kiosses V, Antoniadis A. Genetic heterogeneity of HIV-1 in Greece. Microbes Infect. 2000;2:353–357. - PubMed
    1. Alaeus A, Leitner T, Lidman K, Albert J. Most HIV-1 genetic subtypes have entered Sweden. AIDS. 1997;11:199–202. - PubMed
    1. Barlow K L, Tosswill J H, Clewley J P. Analysis and genotyping of PCR products of the Amplicor HIV-1 kit. J Virol Methods. 1995;52:65–74. - PubMed
    1. Carr J K, Salminen M O, Albert J, Sanders-Buell E, Gotte D, Birx D L, McCutchan F E. Full genome sequences of human immunodeficiency virus type 1 subtypes G and A/G intersubtype recombinants. Virology. 1998;247:22–31. - PubMed
    1. Clevenbergh P, Durant J, Halfon P, del Giudice P, Mondain V, Montagne N, Schapiro J M, Boucher C A, Dellamonica P. Persisting long-term benefit of genotype-guided treatment for HIV-infected patients failing HAART. The Viradapt Study: week 48 follow-up. Antivir Ther. 2000;5:65–70. - PubMed