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. 2001 Sep;132(3):427-9.
doi: 10.1016/s0002-9394(01)01021-2.

Upregulation of pigment epithelium-derived factor after laser photocoagulation

N Ogata  1 , J Tombran-Tink, N Jo, D Mrazek, M Matsumura
Affiliations

Upregulation of pigment epithelium-derived factor after laser photocoagulation

N Ogata et al. Am J Ophthalmol. 2001 Sep.

Abstract

Purpose: To determine the changes in the expression of pigment epithelium-derived factor in cultured human retinal pigment epithelial cells and rat retinas after laser photocoagulation.

Methods: Experimental study of laser photocoagulation on human retinal pigment epithelial cells in culture and on adult rats. Reverse transcription-polymerase chain reaction and semiquantitative polymerase chain reaction analysis were used.

Results: After photocoagulation, the mRNA expression of pigment epithelium-derived factor was upregulated in human retinal pigment epithelial cells at 6 hours and then gradually decreased. Compared with controls, significantly higher levels of pigment epithelium-derived factor were observed in rat retinas from 6 to 24 hours after laser photocoagulation (P <.005), and they were still higher than before photocoagulation at 2 weeks.

Conclusion: An upregulation of pigment epithelium-derived factor in retinal pigment epithelial cells and in the retina after photocoagulation suggests that pigment epithelium-derived factor plays a role in inhibiting neovascularization by its antiangiogenic activity.

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