doi: 10.1007/BF01404744.
Cell culture for three-dimensional modeling in rotating-wall vessels: an application of simulated microgravity
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- PMID: 11541102
- DOI: 10.1007/BF01404744
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Cell culture for three-dimensional modeling in rotating-wall vessels: an application of simulated microgravity
J Tissue Cult Methods.
1992.
Abstract
High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 X 10(7) cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.
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