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. 1975;22(2):163-8.

Role of Mn2+ in the reaction of polynucleotide phosphorylase with 2'-O-methylated substrates

J A Siedlecki, B Zmudzka
  • PMID: 1154944

Role of Mn2+ in the reaction of polynucleotide phosphorylase with 2'-O-methylated substrates

J A Siedlecki et al. Acta Biochim Pol. 1975.

Abstract

Polymerization of 2'-O-methylcytidine-5'-diphosphate (CmDP) with polynucleotide phosphorylase in the presence of Mn2+ proceeds with 65% yield after 72 h, and in the presence of Mg2+ the yield does not exceed 10%. Phosphorolysis of poly 2'-O-methylcytidylic acid and poly 2'-O-methyluridylic acid, as well as exchange of the beta-phosphate group of CmDP in the presence of Mn2+ and Mg2+, proceed with a yield of only a few percent. A possible mechanism of Mn2+ action on CmDP polymerization is discussed.

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