Tracing of surfactants in the biological wastewater treatment process and the identification of their metabolites by flow injection-mass spectrometry and liquid chromatography-mass spectrometry and -tandem mass spectrometry

Abstract

Results of aerobic biodegradation of alkyl ethoxylates (AEOs), of nonylphenol polyethoxylates (NPEOs), and of NPEO derivatives (sulfonates and sulfates), as well as anaerobic NPEO biodegradation monitored by flow injection analysis (FIA) or liquid chromatographic separation (LC) in combination with mass (MS) and tandem mass spectrometry (MS-MS) are presented. The application of visual pattern recognition in the FIA-MS mode showed quite different degradation pathways for C13-AEOs, so that aldehyde compounds as metabolites could be confirmed by collision-induced dissociation for the first time. Methyl ethers of AEO compounds were found to be persistent under aerobic conditions, while NPEO degradation resulted in nonylphenol polyether carboxylates. FIA- and LC-MS proved that NPEO derivatives used as anionic surfactants were either non-biodegradable (nonylphenol diethoxy sulfonate) or were primarily degraded (nonylphenol polyethoxy sulfates) into compounds of the same molar masses yet of different retention behaviour. Anaerobic degradation of NPEOs led to the generation of nonylphenols, which was confirmed by GC-MS.