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. 2001 Oct;75(20):10000-4.
doi: 10.1128/JVI.75.20.10000-10004.2001.

Arginine-glycine-aspartic acid motif is critical for human parechovirus 1 entry

Y Boonyakiat  1 , P J Hughes, F Ghazi, G Stanway
Affiliations

Arginine-glycine-aspartic acid motif is critical for human parechovirus 1 entry

Y Boonyakiat et al. J Virol. 2001 Oct.

Abstract

The human parechovirus 1 RGD motif in VP1 was studied by mutagenesis. An RGD-to-RGE change gave only revertant viruses with a restored RGD, while deletion of GD was lethal and nonrevertable. Mutations at the +1 and +2 positions had some effect on growth properties and a +1 M-to-P change was lethal. These studies indicate that the RGD motif plays a critical role in infectivity, presumably by interacting with integrins, and that downstream amino acids can have an influence on function.

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Figures

FIG. 1
FIG. 1
Amino acid sequence comparison of the VP1 C-terminal region of representative strains of HPeV1, HPeV2, and the enteroviruses CAV9 and EV9 (4, 12, 16, 33, 51). The sequences of the RGD-containing G-H loop regions of representatives of each FMDV serotype are also shown (1, 6, 8, 45, 46).
See this image and copyright information in PMC

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